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    <title>Recitations on MIT OpenCourseWare</title>
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    <description>Recent content in Recitations on MIT OpenCourseWare</description>
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      <title>dev1_recnotes_fx.pdf</title>
      <link>/resources/dev1_recnotes_fx/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
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      <description>This resource includes information about development: from a single-cell zygote to a multi-organ organism, advatages of zebrafish as a model organism in developmental biology, study development at morphological and molecular level and RNA vs DNA.</description>
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      <title>dev2_recnotes_fx.pdf</title>
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      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
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      <description>This resource includes information about RNA, denaturants, embryonic staging bymorphological criteria, teratogen and teratogenesis, how does teratogen work and teratogen vs mutagen.</description>
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    <item>
      <title>dev3_recnotes_fx.pdf</title>
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      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
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      <description>This resource includes information about RNA fixation, how is RNA/DNA transferred?, UV crosslinking and probe labeling vs PCR.</description>
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    <item>
      <title>dev4_recnotes_fx.pdf</title>
      <link>/resources/dev4_recnotes_fx/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
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      <description>This resource includes information about purpose of pre-hyb, Pre-hyb/hyb buffer, Hybridization temperature and Zebrafish development observation.</description>
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    <item>
      <title>dev5_recnotes_fx.pdf</title>
      <link>/resources/dev5_recnotes_fx/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/dev5_recnotes_fx/data.json</guid>
      <description>This resource includes information about zebrafish development and teratogenesis and LiCl post fertilization.</description>
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    <item>
      <title>gen_mod_overview.pdf</title>
      <link>/resources/gen_mod_overview/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/gen_mod_overview/data.json</guid>
      <description>This resource provides information about the overview of microbial genetics module.</description>
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    <item>
      <title>gen1_recnts_esfn.pdf</title>
      <link>/resources/gen1_recnts_esfn/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/gen1_recnts_esfn/data.json</guid>
      <description>This resource provides information about sterile techniques, media, growth curve and indicator plates.</description>
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    <item>
      <title>gen2_rn_estr_fn.pdf</title>
      <link>/resources/gen2_rn_estr_fn/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/gen2_rn_estr_fn/data.json</guid>
      <description>This resource provides information about overview of genetics module and mutagenesis strategy.</description>
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    <item>
      <title>gen2_rn_eswf_fn.pdf</title>
      <link>/resources/gen2_rn_eswf_fn/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/gen2_rn_eswf_fn/data.json</guid>
      <description>This resource includes information about overview of genetics module.</description>
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    <item>
      <title>gen3_recnotes_es.pdf</title>
      <link>/resources/gen3_recnotes_es/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/gen3_recnotes_es/data.json</guid>
      <description>This resource includes information about characterization of putative Ara-mutants, lacZ expression (to produce ?-galactosidase) and lambda titration.</description>
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    <item>
      <title>gen4_recnts_esfn.pdf</title>
      <link>/resources/gen4_recnts_esfn/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/gen4_recnts_esfn/data.json</guid>
      <description>This resource includes information about MOI calculations, generalized transduction, type of MOI required for each experiment in the GEN module, and why and chloroform.</description>
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    <item>
      <title>gen5_recnts_esfn.pdf</title>
      <link>/resources/gen5_recnts_esfn/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/gen5_recnts_esfn/data.json</guid>
      <description>This resource includes information about homologous recombination, generalized transduction: stabilization and mapping and choosing controls for Day 6 patching.</description>
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    <item>
      <title>gen6_recnts_esfn.pdf</title>
      <link>/resources/gen6_recnts_esfn/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/gen6_recnts_esfn/data.json</guid>
      <description>This resource includes information about genetic linkage, metabolism: catabolism vs. anabolism and cotransduction mapping.</description>
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    <item>
      <title>mut_strtg_cendog.pdf</title>
      <link>/resources/mut_strtg_cendog/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/mut_strtg_cendog/data.json</guid>
      <description>This resource provides information about the central dogma of biology and how it relates to the Gen Module.</description>
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    <item>
      <title>pbc1_nts_aa_sp5.pdf</title>
      <link>/resources/pbc1_nts_aa_sp5/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/pbc1_nts_aa_sp5/data.json</guid>
      <description>This resource includes information about why purify proteins, why does purification work, ?-galactosidase intro, module overview and ?-galactosidase activity assay.</description>
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    <item>
      <title>pbc2_nts_aa_sp5.pdf</title>
      <link>/resources/pbc2_nts_aa_sp5/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/pbc2_nts_aa_sp5/data.json</guid>
      <description>This resource includes information about column chromatography, size exclusion chromatography, pK&amp;rsquo;s of amino acids and anion exchange chromatography.</description>
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    <item>
      <title>pbc3_nts_aa_sp5.pdf</title>
      <link>/resources/pbc3_nts_aa_sp5/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/pbc3_nts_aa_sp5/data.json</guid>
      <description>This resource includes information about affinity chromatography.</description>
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    <item>
      <title>pbc4_nts_aa_sp5.pdf</title>
      <link>/resources/pbc4_nts_aa_sp5/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/pbc4_nts_aa_sp5/data.json</guid>
      <description>This resource includes information about quantitative and qualitative assessment of purity/concentration, sodium dodecyl sulphate-poly acrylamide gel electrophoresis, ?-mercaptoethanol, glycerol, bromophenol blue dye, tris and BSA standard curve.</description>
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    <item>
      <title>pbc5_nts_aa_sp5.pdf</title>
      <link>/resources/pbc5_nts_aa_sp5/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
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      <description>This resource includes information about western blotting, antibodies, nitrocellulose membrane and alkaline phosphatase.</description>
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    <item>
      <title>pbc6_nts_aa_sp5.pdf</title>
      <link>/resources/pbc6_nts_aa_sp5/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
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      <description>This resource includes information about enzyme kinetics and its characteristics.</description>
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    <item>
      <title>rdm_overview.pdf</title>
      <link>/resources/rdm_overview/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/rdm_overview/data.json</guid>
      <description>This resource includes information about subclonning and expression of GFP and PCR analysis of ara mutant.</description>
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    <item>
      <title>rdm1_lal.pdf</title>
      <link>/resources/rdm1_lal/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/rdm1_lal/data.json</guid>
      <description>This resource includes information about module overview, restriction mapping, restriction digests and agarose gel electrophoresis.</description>
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    <item>
      <title>rdm2_recnts_lal.pdf</title>
      <link>/resources/rdm2_recnts_lal/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/rdm2_recnts_lal/data.json</guid>
      <description>This resource includes information about CIP (calf intestinal phosphatase) treatment of vector, gel purification/isolation, ligation and inoculate ara-mutant.</description>
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    <item>
      <title>rdm3_recnts_lal.pdf</title>
      <link>/resources/rdm3_recnts_lal/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/rdm3_recnts_lal/data.json</guid>
      <description>This resource includes information about subcloning project-transformation and PCR polymerase chain reaction.</description>
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    <item>
      <title>rdm4_recnts_lal.pdf</title>
      <link>/resources/rdm4_recnts_lal/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/rdm4_recnts_lal/data.json</guid>
      <description>This resource includes information about miniprep protocol and PCR polymerase chain reaction.</description>
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    <item>
      <title>rdm5_recnts_lal.pdf</title>
      <link>/resources/rdm5_recnts_lal/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/rdm5_recnts_lal/data.json</guid>
      <description>This resource includes information about examples of gel from PCR analysis of ara mutants and subcloning project.</description>
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    <item>
      <title>rdm6_allrecnotes.pdf</title>
      <link>/resources/rdm6_allrecnotes/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/rdm6_allrecnotes/data.json</guid>
      <description>This resource includes information about primer design, where do they come from and how to design them.</description>
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    <item>
      <title>Recitations</title>
      <link>/pages/recitations/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/pages/recitations/data.json</guid>
      <description>Laboratory sessions begin with a short recitation reviewing basic guidelines and principles for the assigned experiments of that day.
A sessionwise listing of downloadable notes appear in the table below, courtesy of and used with permission from the following contributors: Genetics module notes by Eric Sullivan; Protein Biochemistry module notes by Anonymous; Recombinant DNA Methods notes by Laura Anne Lowery; Recombinant DNA Methods Overview notes by Michael Lawrence; Development notes by Fang Xie.</description>
    </item>
    
    <item>
      <title>sds_pg_protocols.pdf</title>
      <link>/resources/sds_pg_protocols/data.json</link>
      <pubDate>Mon, 01 Jan 0001 00:00:00 +0000</pubDate>
      
      <guid>/resources/sds_pg_protocols/data.json</guid>
      <description></description>
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