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9.013J | Spring 2008 | Graduate

# Cell and Molecular Neurobiology

### Textbook

Levitan, Irwin B., and Leonard K. Kaczmarek. The Neuron: Cell & Molecular Biology. 3rd ed. New York, NY: Oxford University Press, 2001. ISBN: 9780195145236.

List of optional texts (PDF)

### Calendar of Reading Assignments

Readings for this course include readings from the class textbook as well as from a variety of peer-reviewed journals.

LEC # | TOPICS | READINGS | RECOMMENDED REVIEWS/READINGS
--- | --- | --- | ---

1 | Ion channels I | Chapters 5 and 7 |

2 | Ion channels II | Chapters 5 and 7 | Choe, Senyon. “Potassium Channel Structures.” Neuroscience 3 (February 2002): 115-121. Grottesi, Alessandro, Zara A. Sands, and Mark S. P. Sansom. “Potassium Channels: Complete and Undistorted.” Current Biology 15, no. 18 (September 20, 2005): R771-R774.

3 | Receptors I | Chapters 11 and 12 | Lefkowitz, Robert J. “Historical Review: A Brief History and Personal Retrospective of Seven-transmembrane Receptors.” TRENDS in Pharmacological Sciences 25, no. 8 (August 2004): 413-422. Strader, Catherine D., Tung Ming Fong, Michael R. Tota, and Underwood Dennis. “Structure and Function of G Protein-coupled Receptors.” Annu Rev Biochem 63 (1994): 101-32. Gainetdinov, Raul R., Richard T. Premont, Laura M. Bohn, et al. “Desensitization of G Protein–coupled Receptors and Neuronal Functions.” Annu Rev Neurosci 27 (2004):107-44. Clapham, David E. “TRP Channels as Cellular Sensors.” Nature 426, no. 4 (December 2003): 517-524. Ashmore, Jonathan. “Channel at the Hair’s End.” Nature 432, no. 9 (December 2004): 685-686. Barritt, Greg Barritt, and Grigori Rychkov. “TRPs as Mechanosensitive Channels.” Nature Cell Biology 7, no. 2 (February 2005): 105-107. Corey, David P. “What is the Hair Cell Transduction Channel?” J Physiol 576, no. 1 (2006): 23-28.

4 | Receptors II | Chapters 11 and 12 | Lefkowitz, Robert J, and Erin J Whalen. “β-Arrestins: Traffic Cops of Cell Signaling.” Current Opinion in Cell Biology 16 (2004): 162-168. Shenoy, Sudha K., Patricia H. McDonald, Trudy A. Kohout, et al. “Regulation of Receptor Fate by Ubiquitination of Activated β2 -Adrenergic Receptor and β-Arrestin.” Science 294, no. 9 (November 2001): 1307-1313. Gilman, Alfred G. “G Proteins and Regulation of Adenylyl Cyclase.” Bioscience Reports 15, no. 2 (April 1995): 65-97. Neer, Eva J. “Heterotrimeric G Proteins: Review Organizers of Transmembrane Signals.” Cell 80 (January 27, 1995): 249-257. Pierce, Kristen L., Richard T. Premont, and Robert J Lefkowitz. “Seven-Transmembrane Receptors.” Molecular Cell Biology 3 (September 2002): 639-650. von Zastrow, Mark, et al. “Regulated Endocytosis of Opioid Receptors: Cellular Mechanisms and Proposed Roles in Physiological Adaptation to Opiate Drugs.” Current Opinion in Neurobiology 13 (2003): 348-353.

5 | Introduction; basics of electrophysiology | Chapters 1 and 4 | Nowak L., P. Bregestovski, and P. Ascher. “Magnesium Gates Glutamate-activated Channels in Mouse Central Neurons.” Nature 307 (February 1984): 462-465.

6 | Modulation of receptor activity | Chapter 13, pp. 315-331 |

7 | Protein targeting, signaling complexes |  |

8 | Neuronal polarity / subcellular specialization | Chapter 2 | Jiang, Hui, Guo, Wei, Liang, Xinhua, et al. “Both the Establishment and the Maintenance of Neuronal Polarity Require Active Mechanisms: Critical Roles of GSK-3β and Its Upstream Regulators.” Cell 120 (January 14, 2005): 123-135. Ye, Bing, et al. “Growing Dendrites and Axons Differ in Their Reliance on the Secretory Pathway.” Cell 130 (August 24, 2007): 717-729.

9 | Repertoire of neuron interactions | Chapters 2, 3, 8, and 16, pp. 395-406 |

10 | Neurogenesis | Chapter 15 | Buchman, Joshua J., and Li-Huei Tsai. “Spindle Regulation in Neural Precursors of Flies and Mammals.” Neuroscience 8 (February 2007): 89-100. Ayala, Ramsés, Tianzhi Shu, and Li-Huei Tsai. “Trekking across the Brain: The Journey of Neuronal Migration.” Cell 128 (January 12, 2007): 29-43.

11 | Presynaptic mechanisms | Chapter 9 | Jahn, Reinhard, Thorsten Lang, and Thomas C. Sudhof. “Membrane Fusion.” Cell 112 (February 21, 2003): 519-533. Rizo, Josep, and Thomas C. Sudhof. “Snares and Munc18 in Synaptic Vesicle Fusion.” Neuroscience 3 (August 2002): 641-653. Edwards, Robert H. “The Neurotransmitter Cycle and Quantal Size.” Neuron 55 (September 20, 2007): 835-858. Galli, Thierry, and Volker Haucke. “Cycling of Synaptic Vesicles: How Far? How Fast!” Sci STKE, no. 88 (June 26, 2001): 1-10. Stevens, Charles F. “Neurotransmitter Release at Central Synapses.” Neuron 40 (October 9, 2003): 381-388. Yoshihara, Motojiro, Bill Adolfsen, and Troy J. Littleton. “Is Synaptotagmin the Calcium Sensor?” Current Opinion in Neurobiology 13 (2003): 315-323. Koh, Tong-Wey Koh, and Hugo J. Bellen. “Synaptotagmin I, a Ca2+ sensor for Neurotransmitter Release.” TRENDS in Neurosciences 26, no. 8 (August 2003): 413-422.

| Mid-term exam |

12 | Molecular manipulation of neural circuits |  | Tervo, Dougal, and Alla Y. Karpova. “Rapidly Inducible, Genetically Targeted Inactivation of Neural and Synaptic Activity In vivo.” Current Opinion in Neurobiology 17 (2007): 581-586.

13 | Axon guidance |  | Tessier-Lavigne, Marc, and Corey S. Goodman. “The Molecular Biology of Axon Guidance.” Science 274, no. 5290 (November 15, 1996): 1123-1133.

14 | Synapse formation/elimination | Chapter 18 |

15 | Trophic factors | Chapter 16, pp. 408-427 | Chao, Moses V. “Neurotrophins and their Receptors: A Convergence Point for many Signaling Pathways.” Neuroscience 4 (April 2003): 299-309.

16 | Postsynaptic mechanisms; LTP/LTD | Chapter 20, pp. 558-570 | Sheng, Morgan, et al. “Postsynaptic Signaling and Plasticity Mechanisms.” Science 298 (October 25, 2002): 776-780. Merrill, Michelle A., Yucui Chen, Stefan Strack, et al. “Activity-driven Postsynaptic Translocation of CaMKII.” TRENDS in Pharmacological Sciences 26, no. 12 (December 2005): 645-653. Malenka, Robert C., and Mark F. Bear. “LTP and LTD: An Embarrassment of Riches.” Neuron 44 (September 30, 2004): 5-21. Malenka, Robert C. “The Long-term Potential of LTP.” Neuroscience 4 (November 2003): 923-926. Renger, John J., Christophe Egles, and Guosong Liu. “A Developmental Switch in Neurotransmitter Flux Enhances Synaptic Efficacy by Affecting AMPA Receptor Activation.” Neuron 29 (February 2001): 469-484. Kelleher, Raymond J., Arvind Govindarajan, and Susumu Tonegawa. “Translational Regulatory Mechanisms in Persistent Forms of Synaptic Plasticity.” Neuron 44 (September 30, 2004): 59-73. Frey, Uwe, and Richard G. M. Morris. “Synaptic Tagging: Implications for Late Maintenance of Hippocampal Long-term Potentiation.” TINS 21, no. 5 (1998): 181-188. Martin, Kelsey C., and Kenneth S. Kosik. “Synaptic Tagging - Who’s it?” Neuroscience 3 (October 2002): 813-820.

17 | Waves and chemoaffinity |  | Wilkinson, David G. “Multiple Roles of EPH Receptors and Eprins in Neural Development.” Neuroscience 2 (March 2001): 155-164. Feldheim, David A., Young-In Kim, and Andrew D. Bergeman, et al. “Genetic Analysis of Ephrin-A2 and Ephrin-A5 Shows Their Requirement in Multiple Aspects of Retinocollicular Mapping.” Neuron 25 (March 2000): 563-574. Klein, Rüdiger. “Excitatory Eph Receptors and Adhesive Ephrin Ligands.” Current Opinion in Cell Biology 13 (2001): 196-203. Simon, David K., and Dennis D. M. O’Leary. “Development of Topographic Order in the Mammalian Retinocollicular Projection.” The Journal of Neuroscience 12, no. 4 (April 1992): 1212-1232. Simon, David K., Glen T. Pruskyt, Dennis D. M. O’Leary, et al. “N-Methyl-D-Aspartate Receptor Antagonists Disrupt the Formation of a Mammalian Neural Map.” Proc Natl Acad Sci 89 (November 1992): 10593-10597. Zhou, Jimmy Z. “Direct Participation of Starburst Amacrine Cells in Spontaneous Rhythmic Activities in the Developing Mammalian Retina.” The Journal of Neuroscience 18, no. 11 (June 1, 1998): 4155-4165. Butts, Daniel A. “Retinal Waves: Implications for Synaptic Learning Rules during Development.” The Neuroscientist 8, no. 3 (2002): 243-253. Butts, Daniel A., and Daniel S. Rokhsar. “The Information Content of Spontaneous Retinal Waves.” The Journal of Neuroscience 21, no. 3 (February 1, 2001): 961-973. Söhl, Goran, Stephan Maxeiner, and Klaus Willecke. “Expression and Functions of Neuronal Gap Junctions.” Neuroscience 6 (March 2005): 191-200. Torborg, Christine L, Kristi A Hansen, and Marla B Feller. “High Frequency, Synchronized Bursting Drives Eye-specific Segregation of Retinogeniculate Projections.” Nature Neuroscience 8, no. 1 (January 2005): 72-78. Mann, Fanny, William A. Harris, and Christine E. Holt. “New Views on Retinal Axon Development: A Navigation Guide.” Int J Dev Biol 48 (2004): 957-964.

18 | Two faces of GABA |  | Owens, David F., and Arnold R. Kriegstein. “Is There More to Gaba than Synaptic Inhibition?” Neuroscience 3 (September 2002): 715-727.

19 | Hodgkin and Huxley (lecture 1) |  | Hodgkin, A. L., A. F. Huxley, and B. Katz. “Measurement of Current-voltage Relations in the Membrane of the Giant Axon of Loligo.” J Physiol 116 (1952): 424-448. Hodgkin, A. L., and A. F. Huxley. “Currents Carried by Sodium and Potassium Ions through the Membrane of the Giant Axon of Loligo.” J Physiol 116 (1952): 449-472. ———. “The Components of Membrane Conductance in the Giant Axon of Loligo.” J Physiol 116 (1952): 473-496. ———. “The Dual Effect of Membrane Potential on Sodium Conductance in the Giant Axon of Loligo.” J Physiol 116 (1952): 497-506. ———. “A Quantitative Description of Membrane Current and Its Application to Conduction and Excitation in Nerve.” J Physiol 117 (1952): 500-544.

20 | Hodgkin and Huxley (lecture 2) | Chapters 19 and 20 |

21 | Aplysia learning |  | Bailey, Craig H., Eric R. Kandel, and Kausik Si. “The Persistence of Long-term Memory: A Molecular Approach to Self-sustaining Changes in Learning-induced Synaptic Growth.” Neuron 44 (September 30, 2004): 49-57.

22 | Drosophila learning |  | Keene, Alex C., and Scott Waddell. “Drosophila Olfactory Memory: Single Genes to Complex Neural Circuits.” Neuroscience 8 (May 2007): 341-354.

23 | Drosophila circadian rhythms |  | Nitabach, Michael N., and Paul H. Taghert. “Organization of the Drosophila Circadian Control Circuit.” Current Biology 18, no. 2 (January 22, 2008): R84-R93.

24 | Epilepsy |  | Steinlein, Ortrud K. “Genetic Mechanisms that Underlie Epilepsy.” Neuroscience 5 (May 2004): 400-408.

| Final exam |

## Course Info

##### Instructors

- Prof. Martha Constantine-Paton
- Prof. Morgan Sheng
- Prof. William Quinn

##### Departments

- Brain and Cognitive Sciences
- Biology

##### As Taught In

Spring 2008

##### Level

Graduate

##### Topics

- Science
  - Biology
    - Cell Biology
    - Molecular Biology
    - Neurobiology

##### Learning Resource Types

assignment Presentation Assignments

Browse Resources

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