MIT Open Access Articles

Adaptation of Aglycosylated Monoclonal Antibodies for Improved Production in Komagataella phaffii

Yang, Yuchen; Dalvie, Neil C; Brady, Joseph R; Naranjo, Christopher A; Lorgeree, Timothy; e.a. (Wiley, 2025-02)

Monoclonal antibodies (mAbs) are a major class of biopharmaceuticals manufactured by well-established processes using Chinese Hamster Ovary (CHO) cells. Next-generation biomanufacturing using alternative hosts like ...
Modulation of antigen delivery and lymph node activation in nonhuman primates by saponin adjuvant saponin/monophosphoryl lipid A nanoparticle

Yousefpour, Parisa; Zhang, Yiming J; Maiorino, Laura; Melo, Mariane B; Arainga Ramirez, Mariluz A; e.a. (Oxford University Press, 2024-11-25)

Saponin-based vaccine adjuvants are potent in preclinical animal models and humans, but their mechanisms of action remain poorly understood. Here, using a stabilized HIV envelope trimer immunogen, we carried out studies ...
Vaccines combining slow release and follicle targeting of antigens increase germinal center B cell diversity and clonal expansion

Rodrigues, Kristen A; Zhang, Yiming J; Lam, Jonathan; Aung, Aereas; Morgan, Duncan M; e.a. (American Association for the Advancement of Science, 2025-06-18)

Vaccine adjuvants play important roles in shaping the humoral response to immunization. Here, we analyzed mechanisms of action of a clinically relevant combination adjuvant strategy, where phosphoserine (pSer)–tagged ...